Based on the POI data of Shanghai’ s digital culture industry, using ArcGIS software, combined with nuclear density analysis, spatial autocorrelation analysis， standard deviation ellipse analysis and other methods, this paper analyzes the spatial agglomeration characteristics and spatial differentiation pattern of Shanghai’ s digital culture industry, and discusses the influencing factors and interactive mechanism of spatial differentiation of Shanghai’s digital culture industry. The research finds that ：（1） Shanghai’s digital culture industry has a high overall level of agglomeration, and the degree of regional agglomeration is significantly different. （2） Shanghai’s digital culture industry is spatially non-random and has obvious spatial autocorrelation, showing a ladder pattern of differentiation from south to north. （3） There are differences in the degree of agglomeration of various categories of digital culture industry in Shanghai, among which the degree of agglomeration of application software development is the highest and that of broadcasting and television is the lowest.